Fluorescence Determination of Hydrogen Peroxide Using Hemoglobin as a Mimetic Enzyme of Peroxidase.

Fluorescence determination of hydrogen peroxide using hemoglobin as a mimetic enzyme of peroxidase.

Hydrogen Peroxide Biosensors Based on Horseradish Peroxidase and Hemoglobin

Hydrogen peroxide (H2O2) plays a pivotal role in various industrial applications [1]. It is an essential mediator in pharmaceutical, clinical, and environmental research. In addition, it is a byproduct of highly selective oxidases and an important contaminant in several industrial products and wastes [2]. Therefore, a reliable and economical method for the determination of H2O2 is of great sign...

Hemoglobin can attenuate hydrogen peroxide-induced oxidative stress by acting as an antioxidative peroxidase.

Hemoglobin is considered a potentially toxic molecule when released from erythrocytes during hemolysis, inflammation, or tissue injury. The mechanisms of toxicity involve reduced nitric oxide bioavailability and oxidative processes both occurring at the heme prosthetic groups. When the endogenous oxidant H(2)O(2) reacts with Hb, transient radicals are generated during the peroxidative consumpti...

GasniGraphene-manganase oxide nanocomposite as a hydrogen peroxidase sensor

A feasible and fast method to fabricate hydrogen peroxide sensor was investigated by graphene-manganase nanocomposite carbone paste electrode. In the present work, in first step, the graphene was synthesized by chemical method and in second step, manganese oxide nanoparticle was doped on graphene. graphene-manganase nanocomposite was characterized by FTIR and SEM. The nanocomposite shows a high...

GasniGraphene-manganase oxide nanocomposite as a hydrogen peroxidase sensor

A feasible and fast method to fabricate hydrogen peroxide sensor was investigated by graphene-manganase nanocomposite carbone paste electrode. In the present work, in first step, the graphene was synthesized by chemical method and in second step, manganese oxide nanoparticle was doped on graphene. graphene-manganase nanocomposite was characterized by FTIR and SEM. The nanocomposite shows a high...